Sack and Yarov-Yarovoy:Protocols

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(Tentagel Azide Beads)
(Fluorophore Labeling)
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[[Sack: Agar Bridge Formation| Agar Bridge Formation]][  .]<br>
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][  .]<br>
==Fluorophore Labeling==
[[Sack: GxTx Purification|GxTx Purification 11/22/11]][  .]<br>
[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]][  .]<br>
[[Sack: Maleimide Labeling| Maleimide Labeling 11/08/11]][  .]<br>
[[Sack: Maleimide Labeling TAMRA| Maleimide Labeling TAMRA 10/08/12]][  .]<br>
==General Lab==
==General Lab==
[[Sack: Acidwash|Acidwash 03/22/11]][  .]<br>
[[Sack: Acidwash|Acidwash 03/22/11]][  .]<br>

Revision as of 15:03, 25 October 2012


Cell Culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Fluorescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.


Agar Bridge Formation.

General Lab

Acidwash 03/22/11.


BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.


Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.

Molecular Biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

Tentagel Azide Beads

Bead Cell Binding 05/15/12.
Beads Alykne Azide BTTAA 05/16/12.

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