Sack and Yarov-Yarovoy:Protocols

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Revision as of 16:24, 12 January 2013 by Daniel Clay Austin (talk | contribs) (Sack:Protocols moved to Sack and Yarov-Yarovoy:Protocols: This page needs to be moved since the Sack and Yarov-Yarovoy Labs have decided to collaborate on a single openwetware page, due to the extensive amount of work these labs do together.)
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general lab

Acidwash 03/22/11.

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophys

Agar Bridge Formation.

molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.
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