Sack and Yarov-Yarovoy:Protocols

From OpenWetWare

Revision as of 21:21, 10 October 2012 by Jon Sack (Talk | contribs)
Jump to: navigation, search


Cell Culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Flourescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.


Agar Bridge Formation.

Flourophore Labeling

GxTx Purification 11/22/11.
Alyknefluor Azide Beads 11/11/11.
Maleimide Labeling 11/08/11.
Maleimide Labeling TAMRA 10/08/12.

General Lab

Acidwash 03/22/11.


BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.


Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11[1]

Molecular Biology

Bacteria Transformation (One Shot Top Ten Invitrogen.

  • Return to Sack Homepage
Personal tools