Sambu/notebook: Difference between revisions
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Stuff done thus far:<br> | Stuff done thus far:<br> | ||
*Did this experiment to verify that OD and cell dilution factors were linearly related> | *Did this experiment to verify that OD and cell dilution factors were linearly related> | ||
[[Image:graph.jpg|thumb|left|250px]].<br><br><br><br><br><br><br><br><br> | [[Image:graph.jpg|thumb|left|250px]].<br><br><br><br><br><br><br><br><br><br><br> | ||
*Performed assays for the expression of his and strep tags on the E.coli surface. Used denaturation electrophoresis for this a picture is shown below:<br> | *Performed assays for the expression of his and strep tags on the E.coli surface. Used denaturation electrophoresis for this a picture is shown below:<br> |
Revision as of 10:47, 7 July 2007
Stuff done thus far:
- Did this experiment to verify that OD and cell dilution factors were linearly related>
.
- Performed assays for the expression of his and strep tags on the E.coli surface. Used denaturation electrophoresis for this a picture is shown below:
6/27 protein denaturation electrophoresis gel
Description: 6/27 denaturation electrophoresis gel
The rightmost lane is the ladder
lane 1 Ladder
lane 2 OmpA1 + strep before induction with IPTG
lane 3 OmpA1 + his6 before induction
lane 4 OmpA1 + strep after induction
lane 5 OmpA1 + his6 after induction
lane 6 OmpA1 + his6 after induction
lane 7 OmpA1 + strep after induction
lane 8 OmpA1 + his6 before induction
lane 9 OmpA1 + strep before induction
Note that there were 2 copies of each sample: samples in lanes 2 to 5 were denatured for a 2 minute period as per protocol while samples in lanes 6 to 9 were denatured for a 10 minute period, Sammy S.
2. Then went on to investigate the appropriate times to induce expression of the OmpA-fusion protein constructs on the E.coli surface using IPTG
Week 3 gel
Gel 2: induced immediately
Lane 1: Ladder
Lane 2: Strep No IPTG
Lane 3: Strep denatured after 30 min
Lane 4: Strep denatured after 60 min
Lane 5: Strep denatured after 90 min
Lane 6: Strep denatured after 120 min
Lane 7: His No IPTG
Lane 8: His denatured after 30 min
Lane 9: His denatured after 60 min
Lane 10: His denatured after 90 min
Gel 1
Lane 1: Ladder
Lane 2: His 0 min induction
Lane 3: His 30 min induction
Lane 4: His 60 min induction
Lane 5: His 90 min induction
Lane 6: His 120 min induction
Lane 7: Strep 0 min induction
Lane 8: Strep 30 min induction
Lane 9: Strep 60 min induction
Lane 10: Strep 90 min induction
Lane 11: Strep 120 min induction
Lane 12: His 0 min induction 120 min denature