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| align="center" style="background:#f0f0f0;"|'''Personnel''' | | align="center" style="background:#f0f0f0;"|'''Personnel''' |
Revision as of 08:29, 4 August 2008
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<td class="tdboxes"><a href="http://openwetware.org/wiki/iGEM:Cambridge/2008/Notebook/Voltage"><img border="0" src="http://openwetware.org/images/0/00/Vol_logo.JPG" width="256" height="201"></a></td>
<td class="tdboxes"><a href="http://openwetware.org/wiki/iGEM:Cambridge/2008/Notebook/Voltage"><img border="0" src="http://openwetware.org/images/d/d1/Mag_logo.JPG" width="210" height="201"></a></td>
<td class="tdboxes"><a href="http://openwetware.org/wiki/iGEM:Cambridge/2008/Notebook/Voltage"><img border="0" src="http://openwetware.org/images/f/fe/Tur_logo.JPG" width="201" height="201"></a></td>
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width="30%"| | Personnel | Progress | |
Research | |||
Potassium intake | |||
Preventing K+ efflux | |||
Bacterial tolerance for high K+ and turgor
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Ligand gated channels | |||
Media | |||
Preliminary wet work | |||
Extract promoter, RBS and terminator BioBricks from registry
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Internal K+ build-up | |||
PCR Kdp K+ pump gene from E.coli MG1655
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Put Kdp gene under control of stationary phase promoter (osmY, used by MIT 2006 team)
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Transform into wildtype and mutant E.coli strains | |||
Test
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Chassis | |||
Order from Yale
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Test
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Controlled K+ efflux | |||
Design sequence based on GluR0 glutamate-gated K+ channel from Synechocystis PCC 6803 | |||
Send to DNA 2.0 for synthesis | |||
Backup
| |||
Ligate gene into BioBrick plasmid | |||
Transform into chosen chassis | |||
Test
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Measuring voltage | |||
Quantify output using oxygen electrode or glass capillary microelectrode | |||
Medium optimisation | |||
Vary K+ concentrations, using KCl | |||
Vary nutrient levels | |||
Output optimisation | |||
Vary strength of promoters/RBS |