Saponin Lysis of RBCs
Jingyang Chen, Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA, 98109, USA. email@example.com
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Lyse red blood cells while leaving the Plasmodium falciparum parasite intact with it's parasite membrane and parasitophorous vacoule membrane. Typically used right before freezing down parasites for genomic DNA extraction, or for getting rid of hemoglobin right before running a Western Blot on parasite extracts.
- 0.15% Saponin in PBS
- 1X PBS
- 12 mL of Plasmodium falciparum blood culture at 4% hematocrit
- Refrigerated microcentrifuge
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