Sarah Carratt: Week 4

Instructions

Terminology

• Explain the following terms in your own words.

1. The Michaelis-Menten model of enzyme kinetics.
2. The experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten.
3. Chemostat reactor.
4. Exponential growth.

Applying Michealis-Menten Models

• Consider a single substrate being converted to product.

E + S ↔ ES → E + P with an initial substrate concentration of 1.0 and an initial enzyme concentration of 0.05. Use the Michaelis-Menten matlab model to explore the following.

1. Set k₁ = 2.0, k_-1 = 0.0. Plot the substrate dynamics for k₂ = 1.0, k₂ = 2.0, k₂ = 5.0, k₂ = 10.0 all on the same graph to see the effect of this parameter.
2. Set k₁ = 2.0, k_-1 = 1.0. Plot the substrate dynamics for k₂ = 1.0, k₂ = 2.0, k₂ = 5.0, k₂ = 10.0 all on the same graph to see the effect of this parameter.
3. Set k₁ = 2.0, k_-1 = 5.0. Plot the substrate dynamics for k₂ = 1.0, k₂ = 2.0, k₂ = 5.0, k₂ = 10.0 all on the same graph to see the effect of this parameter.

You may use the matlab code provided at my lionshare folder.

Estimating Michaelis-Menten Parameters

• Consider the following data:

[S] = 11.0, 16.7, 20.0, 25.0, 33.3, 50.0

V = 0.00952, 0.01111, 0.01282, 0.01515, 0.01852, 0.02128

Apply the Lineweaver-Burk technique to determine Vmax and K.

Online Sources

• Biology Dictionary

Student Response

Terminology

• Explain the following terms in your own words.

1. The Michaelis-Menten model of enzyme kinetics.
2. The experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten.
3. Chemostat reactor.
4. Exponential growth.

Applying Michealis-Menten Models

• Consider a single substrate being converted to product.

E + S ↔ ES → E + P with an initial substrate concentration of 1.0 and an initial enzyme concentration of 0.05. Use the Michaelis-Menten matlab model to explore the following.

1. Set k₁ = 2.0, k_-1 = 0.0. Plot the substrate dynamics for k₂ = 1.0, k₂ = 2.0, k₂ = 5.0, k₂ = 10.0 all on the same graph to see the effect of this parameter.
2. Set k₁ = 2.0, k_-1 = 1.0. Plot the substrate dynamics for k₂ = 1.0, k₂ = 2.0, k₂ = 5.0, k₂ = 10.0 all on the same graph to see the effect of this parameter.
3. Set k₁ = 2.0, k_-1 = 5.0. Plot the substrate dynamics for k₂ = 1.0, k₂ = 2.0, k₂ = 5.0, k₂ = 10.0 all on the same graph to see the effect of this parameter.

You may use the matlab code provided at my lionshare folder.

Estimating Michaelis-Menten Parameters

• Consider the following data:

[S] = 11.0, 16.7, 20.0, 25.0, 33.3, 50.0

V = 0.00952, 0.01111, 0.01282, 0.01515, 0.01852, 0.02128

Apply the Lineweaver-Burk technique to determine Vmax and K.

Navigation Guide

Individual Assignments

Sarah Carratt: Week 2	Sarah Carratt: Week 6	Sarah Carratt: Week 11
Sarah Carratt: Week 3	Sarah Carratt: Week 7	Sarah Carratt: Week 12
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Class Notes

Sarah Carratt_1.18.11	Sarah Carratt_2.3.11	Sarah Carratt_2.22.11
Sarah Carratt_1.20.11	Sarah Carratt_2.8.11	Sarah Carratt_2.24.11
Sarah Carratt_1.25.11	Sarah Carratt_2.10.11	Sarah Carratt_3.1.11
Sarah Carratt_1.27.11	Sarah Carratt_2.15.11	Sarah Carratt_3.3.11
Sarah Carratt_2.1.11	Sarah Carratt_2.17.11	Sarah Carratt_3.8.11

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