Sarah Carratt: Week 4

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Instructions

TERMINOLOGY: Define the four terms.

MM MODELS: Plot the three examples.

MM PARAMETERS: Apply the Lineweaver-Burk technique to determine Vmax and K.

Online Sources

Student Response

Terminology

Exponential Growth Population Example
  1. The Michaelis-Menten equation is used to mathematically illustrate the biological conversion of a substrate and enzyme to a complex and then to a product.
  2. The Lineweaver-Burk experiment is an example of the Michaelis-Menten equation in action. The results were obtained by manipulating the original equation (ie: taking the reciprocal) and converting the MM equation into mx+b format.
  3. A chemostat reaction is a "test" to find the equilibrium point of a system, where the amount of substance consumed equals the amount produced or the amount into the system equals the amount going out.
  4. Exponential growth looks very similar to ex when graphed (pattern: more product=faster growth).

Applying Michealis-Menten Models

  1. k1 = 2.0, k-1 = 0.0, k2 = 1.0, k2 = 2.0, k2 = 5.0, k2 = 10.0
  2. k1 = 2.0, k-1 = 1.0, k2 = 1.0, k2 = 2.0, k2 = 5.0, k2 = 10.0
  3. k1 = 2.0, k-1 = 5.0, k2 = 1.0, k2 = 2.0, k2 = 5.0, k2 = 10.0

Estimating Michaelis-Menten Parameters

Vmax = 0.031551

K = 27.10932



Navigation Guide

Individual Assignments

Sarah Carratt: Week 2 Sarah Carratt: Week 6 Sarah Carratt: Week 11
Sarah Carratt: Week 3 Sarah Carratt: Week 7 Sarah Carratt: Week 12
Sarah Carratt: Week 4 Sarah Carratt: Week 8 Sarah Carratt: Week 13
Sarah Carratt: Week 5 Sarah Carratt: Week 9 Sarah Carratt: Week 14

Class Assignments

Shared Journal: Week 1 Shared Journal: Week 6 Shared Journal: Week 11
Shared Journal: Week 2 Shared Journal: Week 7 Shared Journal: Week 12
Shared Journal: Week 3 Shared Journal: Week 8 Shared Journal: Week 13
Shared Journal: Week 4 Shared Journal: Week 9 Shared Journal: Week 14
Shared Journal: Week 5 Shared Journal: Week 10

Class Notes

Sarah Carratt_1.18.11 Sarah Carratt_2.3.11 Sarah Carratt_2.22.11
Sarah Carratt_1.20.11 Sarah Carratt_2.8.11 Sarah Carratt_2.24.11
Sarah Carratt_1.25.11 Sarah Carratt_2.10.11 Sarah Carratt_3.1.11
Sarah Carratt_1.27.11 Sarah Carratt_2.15.11 Sarah Carratt_3.3.11
Sarah Carratt_2.1.11 Sarah Carratt_2.17.11 Sarah Carratt_3.8.11

Internal Links

BIOL398-01/S11:Assignments BIOL398-01/S11:People BIOL398-01/S11:Sarah Carratt
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