Sarah Carratt: Week 9
From OpenWetWare
Instructions
Formatting
- Store this journal entry as "username Week 9" (i.e., this is the text to place between the square brackets when you link to this page).
- Create the following set of links. (HINT: you can do all of this easily by adding them to your template and then using the template on your pages.)
- Link to your journal entry from your user page.
- Link back from your journal entry to your user page.
- Link to this assignment from your journal entry.
- Don't forget to add the "BIOL398-01/S11" category to the end of your wiki page.
Things to Read
Article
- Schade et al. (2004) Cold Adaptation in Budding Yeast. Molecular Biology of the Cell 15: 5492–5502.
- Link to PDF version of article: use this one for printing/reading.
- Link to full text in HTML: use this one to get the figures for your PowerPoint.
Background
- Thieringer, H.A., Jones, P.G., and Inouye, M. (1998) Cold shock and adaptation. BioEssays 20: 49–57.
- Aguilera, J., Randez-Gil, F., and Prieto, J.A. (2007) Cold Response in Saccharomyces cerevisiae: New Functions for Old Mechanisms. FEMS Microbiological Reviews 31: 327–341.
- Al-Fageeh, M.B. and Smales, C.M. (2006) Control and Regulation of the Cellular Responses to Cold Shock: the Responses in Yeast and Mammalian Systems. Biochemical Journal 397: 247–259.
Preparation for Journal Club 2
- Make a list of at least 10 biological terms for which you did not know the definitions when you first read the article. Define each of the terms. List the citation(s) for the dictionary(s) you use, providing a URL to the page is fine.
- Write an outline of the article and include the following questions:
- What is the main result presented in this paper?
- What is the importance or significance of this work?
- How did they treat the cells (what experiment were they doing?)
- What strain(s) of yeast did they use? Was the strain haploid or diploid?
- What media did they grow them in? What temperature? What type of incubator? For how long?
- What controls did they use?
- How many replicates did they perform per timepoint?
- What mathematical/statistical method did they use to analyze the data?
- What transcription factors did they talk about?
- Briefly state the result shown in each of the figures and tables.
- Each group of students will be assigned one section of the paper. The group will be responsible for preparing that portion of the PowerPoint presentation and explaining the assigned section and figures in detail to the class.
- Introduction, Figures 1, 2: Sarah, Carmen
- Methods, Figures 3, 4: Alondra, Nick
- Discussion, Figures 5, 6: James
Online Sources
Student Response
Biological Terms
- transient changes: [1]
- reducibly induced: [2]
- isoforms: [3]
- aggregation: [4]
- menadione: [5]
- expression profile: [6]
- Msn2p/Msn4p: [7]
- hyperosmotic shock: [8]
- trehalose: [9]
- glutathione/glutaredoxin system: [10]
Outline
- What is the main result presented in this paper?
- What is the importance or significance of this work?
- How did they treat the cells (what experiment were they doing?)
- What strain(s) of yeast did they use? Was the strain haploid or diploid?
- What media did they grow them in? What temperature? What type of incubator? For how long?
- What controls did they use?
- How many replicates did they perform per timepoint?
- What mathematical/statistical method did they use to analyze the data?
- What transcription factors did they talk about?
- Briefly state the result shown in each of the figures and tables.
Individual Assignments
Class Assignments
Class Notes
Internal Links
BIOL398-01/S11:Assignments | BIOL398-01/S11:People | BIOL398-01/S11:Sarah Carratt |