Sarah Carratt 4.05.11: Difference between revisions
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=Review of Homework= | =Review of Homework= | ||
* | *Homework found on Openwetware: [[Sarah Carratt: Week 11]] | ||
*Original data file can be uploaded on [http://www.lionshare.lmu.edu Lionshare] with permission from both [[User:Kam D. Dahlquist | Dr. Kam D. Dahlquist, Ph.D.]] and myself. For more information, [[Special:Emailuser/Sarah Carratt|email me through OpenWetWare]]. | |||
==Timepoints== | ==Timepoints== | ||
*Schade = 0m, 10m, 30m, 120m, 12h, 60h | *Schade = 0m, 10m, 30m, 120m, 12h, 60h | ||
| Line 14: | Line 15: | ||
***wt (4,5,4,5,5) | ***wt (4,5,4,5,5) | ||
***Dgln3 (4,4,4,4,4) | ***Dgln3 (4,4,4,4,4) | ||
== | ==P Values== | ||
*more than 300 p values significant by chance = part of problem, use as guide only | |||
*multiple testing problem corrected by: | |||
**Bonferroni corrections | |||
**Comparing fold changes and p.values | |||
==Most Significantly Changed Genes== | |||
*NSR1 | |||
* | |||
Revision as of 09:56, 5 April 2011
Review of Homework
- Homework found on Openwetware: Sarah Carratt: Week 11
- Original data file can be uploaded on Lionshare with permission from both Dr. Kam D. Dahlquist, Ph.D. and myself. For more information, email me through OpenWetWare.
Timepoints
- Schade = 0m, 10m, 30m, 120m, 12h, 60h
- wt and Dgln3 = 15, 30, 60, 90, 120
Resaon for Statistics
- Is this computed ratio a true difference or a fluxuation due to noise?
- T statistic to P value
- Things that are part of the equation...
- size of effect, aka, is it a big difference?
- amount of variation
- number of replicates
- Schade (confusing)
- wt (4,5,4,5,5)
- Dgln3 (4,4,4,4,4)
P Values
- more than 300 p values significant by chance = part of problem, use as guide only
- multiple testing problem corrected by:
- Bonferroni corrections
- Comparing fold changes and p.values
Most Significantly Changed Genes
- NSR1
