Sarah Carratt 4.05.11

Review of Homework

• Homework found on Openwetware: Sarah Carratt: Week 11
• Original data file can be uploaded on Lionshare with permission from both Dr. Kam D. Dahlquist, Ph.D. and myself. For more information, email me through OpenWetWare.

Timepoints

• Schade = 0m, 10m, 30m, 120m, 12h, 60h
• wt and Dgln3 = 15, 30, 60, 90, 120

Resaon for Statistics

• Is this computed ratio a true difference or a fluxuation due to noise?
• T statistic to P value
• Things that are part of the equation...
  • size of effect, aka, is it a big difference?
  • amount of variation
  • number of replicates
    • Schade (confusing)
    • wt (4,5,4,5,5)
    • Dgln3 (4,4,4,4,4)

P Values

• more than 300 p values significant by chance = part of problem, use as guide only
• multiple testing problem corrected by:
  • Bonferroni corrections
  • Comparing fold changes and p.values

Most Significantly Changed Genes

• NSR1