Sauer:FAQs

SAUER LAB

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Can we get this in the stock room?

Check out MIT ECAT, VWR page (certificates required). There is a listing of items carried in the stock room (link is on the left side of the page).

Need more specific instructions?

• go to the link above
• click on VWR
• click "Place departmental order" button
• the page may ask you to type your certificate password now; you should do it
• your name should show up now; the space for your building-room number should be blank
• type in your building and room number
• click on the "continue button"
• click on the link "VWR/MIT Stockroom Items in 56-070", which can be found on the top, left side of the screen
• choose and select categories to browse through
• you can also order stock room items directly from this site

What if it's not in the stock room, but VWR carries it?

Order the item through MIT ECAT. You will need an account number (get this from Brenda) and you will need to know the G/L account number to use (if you don't already have a list of these, ask Brenda). You can also use ECAT to order from other companies, like Office Depot and Airgas.

Need more specific instructions?

• go to the link above
• click on VWR
• click "Place departmental order" button
• the page may ask you to type your certificate password now; you should do it
• your name should show up now; the space for your building-room number should be blank
• type in your building and room number
• click on the "continue button"
• shop as you would on any other site and add things to the cart
• check out will take you to the MIT SAP site
• create the requisition: the cost object is you account number and the G/L account should correspond to the type of item you have ordered (see the list); you also need to click on the material group on the left hand side. All of these things must be filled out for each item you order, but you can just fill down from the first item
• send requisition to SAP
• click on requisition number and print

How do I submit samples for DNA sequencing?

Check out the MIT biopolymers site. The facility also does mass spectrometry, peptide synthesis, and a few other things. You can download all of the forms you need from the site and there are instructions on how to retrieve your data. You will need an account number (talk to Brenda) to submit samples. Talk to someone in the lab about our login and password for the DNA sequencing site. Whenever sending things to biopolymers, be sure to give Brenda a copy of your submission sheets. This helps with questions about charges later on. Also, for DNA sequencing, keep a copy for yourself. Data is posted as 1, 2, 3,..., so it is important for you to remember which sample corresponds to each number.

How do I order things that you can't use a credit card for (like needles, syringes, ethanol, radioactivity)?

• If these things can be ordered through VWR, MIT ECAT is the easiest way to get them.
• If you can get them in the stock room (needles, syringes), use an MIT requisition form. These can be found in each room in the bin under the packing slip bin.
• Ethanol is a restricted item, and you need to be on an authorization list to order it. You get onto this list as part of the paperwork Brenda helps you with when joining the lab. So, you can't order ethanol online. You can get ethanol in the solvent stock room with an MIT requisition form (bring carriers! they won't give it to you without them), or you can fill out a requisition form and give it to someone in the stock room or the VWR representative, who has a desk a few doors down from the stock room and it will be delivered.
• For other things (like radioactivity), you need to create a requisition through MIT SAPweb.
  • If you are using this for radioactivity, ask someone who has done it before for help because certain non-obvious things need to be included on the request form.
  • Be sure to use or cancel all requisitions/purchase orders. If you don't, they will come back to haunt you.
  • To create a requisition:

1. Click the "purchasing" tab (at the top of the page).
2. Look on the left side of the page, about halfway down for the "Non-partner Requisitions" section. Click the "External vendor" circle, then the "Generate Req Form" button.
3. Fill in the appropriate information on the requisition request sheet, and then click the "submit requisition" button at the bottom. A confirmation page should come up.
4. Click to display the requisiton. Write down the req number or print out the sheet so you can use it later to get your purchase order.
5. After creating the requisition, check back on the site later to get your purchase order number.
   1. Go to the "purchasing" tab, look at the left under "look-ups", click on "display a purchase order".
6. Use the purchase order number when you call the company to place the order.

Where do we order this from?

• glass plates and other Protean II protein gel supplies: Biorad

• rainbow protein ladders: Amerhsam/GE Healthcare

• PD-10 columns: Amersham/GE Healthcare
  • To order from GE use SciQuest which is also part of Ecat

• PVDF membrane: Millipore

• Amicon ultra centrifugal filter devices ("concentrators"): Millipore

• chemicals: Sigma, VWR

How do I dispose of full waste containers?

1. Cap the full waste container and date the red tag.
2. Place the full container inside the grey bin where the old 90 day area used to be (underneath the hood in 68-565).
3. Go to EHS Chemical collection and request a pick up.
4. Generate a new replacement waste bottle complete with a red tag.