Sauer:Making yeg plates
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(Difference between revisions)
(New page: YEG medium: 0.5% yeast extract 1% NaCl 0.4% glucose supplement with 16 mM p-Cl-Phe for yeg-pcp. pcp is not soluble before you autoclave. **DO NOT AUTOCLAVE THE GLUCOSE, ADD AFTER FROM ST...) |
Current revision (14:50, 18 May 2009) (view source) |
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| Line 1: | Line 1: | ||
YEG medium: | YEG medium: | ||
0.5% yeast extract | 0.5% yeast extract | ||
| + | |||
1% NaCl | 1% NaCl | ||
| + | |||
0.4% glucose | 0.4% glucose | ||
| + | |||
supplement with 16 mM p-Cl-Phe for yeg-pcp. | supplement with 16 mM p-Cl-Phe for yeg-pcp. | ||
| + | |||
pcp is not soluble before you autoclave. | pcp is not soluble before you autoclave. | ||
| Line 11: | Line 15: | ||
YEG-agar: | YEG-agar: | ||
5 g yeast extract | 5 g yeast extract | ||
| + | |||
10 g NaCl | 10 g NaCl | ||
| + | |||
2 g D,L-p-Cl-Phe | 2 g D,L-p-Cl-Phe | ||
| + | |||
15 g agar | 15 g agar | ||
| + | |||
1 L H20 | 1 L H20 | ||
Current revision
YEG medium: 0.5% yeast extract
1% NaCl
0.4% glucose
supplement with 16 mM p-Cl-Phe for yeg-pcp.
pcp is not soluble before you autoclave.
- DO NOT AUTOCLAVE THE GLUCOSE, ADD AFTER FROM STERILE STOCK**
YEG-agar: 5 g yeast extract
10 g NaCl
2 g D,L-p-Cl-Phe
15 g agar
1 L H20
AUTOCLAVE
add 0.4% glucose, antibiotics
