Sauer:Making yeg plates

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(New page: YEG medium: 0.5% yeast extract 1% NaCl 0.4% glucose supplement with 16 mM p-Cl-Phe for yeg-pcp. pcp is not soluble before you autoclave. **DO NOT AUTOCLAVE THE GLUCOSE, ADD AFTER FROM ST...)
Current revision (14:50, 18 May 2009) (view source)
 
Line 1: Line 1:
YEG medium:
YEG medium:
0.5% yeast extract
0.5% yeast extract
 +
1% NaCl
1% NaCl
 +
0.4% glucose
0.4% glucose
 +
supplement with 16 mM p-Cl-Phe for yeg-pcp.
supplement with 16 mM p-Cl-Phe for yeg-pcp.
 +
pcp is not soluble before you autoclave.
pcp is not soluble before you autoclave.
Line 11: Line 15:
YEG-agar:
YEG-agar:
5 g yeast extract
5 g yeast extract
 +
10 g NaCl
10 g NaCl
 +
2 g D,L-p-Cl-Phe
2 g D,L-p-Cl-Phe
 +
15 g agar  
15 g agar  
 +
1 L H20
1 L H20

Current revision

YEG medium: 0.5% yeast extract

1% NaCl

0.4% glucose

supplement with 16 mM p-Cl-Phe for yeg-pcp.


pcp is not soluble before you autoclave.

    • DO NOT AUTOCLAVE THE GLUCOSE, ADD AFTER FROM STERILE STOCK**

YEG-agar: 5 g yeast extract

10 g NaCl

2 g D,L-p-Cl-Phe

15 g agar

1 L H20

AUTOCLAVE

add 0.4% glucose, antibiotics

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