Sauer:Making yeg plates

From OpenWetWare

Revision as of 14:50, 18 May 2009 by Jhdavis (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

YEG medium: 0.5% yeast extract

1% NaCl

0.4% glucose

supplement with 16 mM p-Cl-Phe for yeg-pcp.


pcp is not soluble before you autoclave.

    • DO NOT AUTOCLAVE THE GLUCOSE, ADD AFTER FROM STERILE STOCK**

YEG-agar: 5 g yeast extract

10 g NaCl

2 g D,L-p-Cl-Phe

15 g agar

1 L H20

AUTOCLAVE

add 0.4% glucose, antibiotics

Personal tools