Sauer:Protocols

Protein Expression/Purification

Sauer:ClpX purification

Sauer:His6 ClpX purification

Sauer:ClpP purification

Sauer:SspB purification

Sauer:Expression/purification of 35S-Met proteins

Sauer:Purification of His₆-tagged proteins

Sauer:Lysing E. coli with Lysozymes

Sauer:GFP-H6-ssrA purification

In vitro enzymatic assays

Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:In vitro degradation of GFP-ssrA by ClpAP

Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!

Sauer:ATPase assays

Proteins: in vitro

Sauer:Silver staining

Sauer:bis-Tris SDS-PAGE, the very best

Nucleic acids: in vitro

Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Quantitating nucleic acids

Sauer:Site-directed mutagenesis

Sauer:RNA Purification from E. coli

Sauer:Stitching Genes by PCR

Analysis of proteins or RNA in vivo

Sauer:Western blot

Sauer:Quantitative Western blot

Sauer:Northern blot PROTOCOL NEEDED!

Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED!

Sauer:beta-Galactosidase Assay (A better Miller)

More in vivo goodies

Sauer:Competent cell preparation PROTOCOL NEEDED!

Sauer:P1vir phage transduction

Sauer:Plasmid-born λRed recombineering using dsDNA

Other good stuff

Generation of genomic libraries

Sauer:HPLC protocol Sauer:FPLC protocol