Sauer:Protocols: Difference between revisions
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{{Sauer lab sidebar}} | {{Sauer lab sidebar}} | ||
Please link protocols to the general [[Protocols]] page if appropriate. | |||
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<div style="padding: .4em .9em .9em"> | <div style="padding: .4em .9em .9em"> | ||
==Protein Expression/Purification== | ===Protein Expression/Purification=== | ||
[[Sauer:ClpX purification]] | [[Sauer:ClpX purification]] | ||
[[Sauer:ClpP purification]] | [[Sauer:His6 ClpX purification]] | ||
[[Sauer:ClpP purification]] | |||
[[Sauer:ClpA purification]] | |||
[[Sauer:SspB purification]] | |||
[[Sauer:Expression/purification of 35S-Met proteins]] | [[Sauer:Expression/purification of 35S-Met proteins]] | ||
==''In vitro'' enzymatic assays== | [[Sauer:Purification of His-tagged proteins|Sauer:Purification of His<sub>6</sub>-tagged proteins]] | ||
[[Sauer:Lysing E. coli with Lysozymes]] | |||
[[Sauer:GFP-H6-ssrA purification]] | |||
[[Sauer:GST-sFtsH purification]] | |||
[[Sauer:FtsH purification]] | |||
===''In vitro'' enzymatic assays=== | |||
[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | [[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | ||
[[Sauer:In vitro degradation of GFP-ssrA by ClpAP]] | |||
[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | ||
[[Sauer:ATPase assays]] | [[Sauer:ATPase assays]] | ||
[[Sauer:In vitro peptide degradation by ClpXP]] | |||
==Proteins: ''in vitro''== | ===Proteins: ''in vitro''=== | ||
[[Sauer:Silver staining]] | [[Sauer:Silver staining]] | ||
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[[Sauer:bis-Tris SDS-PAGE, the very best]] | [[Sauer:bis-Tris SDS-PAGE, the very best]] | ||
==Nucleic acids: ''in vitro''== | ===Nucleic acids: ''in vitro''=== | ||
[[Sauer:In vitro transcription with T7 RNA polymerase]] | [[Sauer:In vitro transcription with T7 RNA polymerase]] | ||
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[[Sauer:Phenol/chloroform extraction]] | [[Sauer:Phenol/chloroform extraction]] | ||
[[ | [[Quantitating nucleic acids]] | ||
[[Sauer:Site-directed mutagenesis]] | [[Sauer:Site-directed mutagenesis]] | ||
[[Sauer:RNA Purification from E. coli]] | |||
[[Sauer:Stitching Genes by PCR]] | |||
</div> | </div> | ||
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<div style="clear: right; text-align: left; float: left; padding: .4em .9em .9em"> | <div style="clear: right; text-align: left; float: left; padding: .4em .9em .9em"> | ||
==Analysis of proteins or RNA ''in vivo''== | ===Analysis of proteins or RNA ''in vivo''=== | ||
[[Sauer:Western blot]] | [[Sauer:Western blot]] | ||
[[Sauer:Quantitative Western blot]] | [[Sauer:Quantitative Western blot]] | ||
[[Sauer:Northern blot]] PROTOCOL NEEDED! | [[Sauer:Northern blot]] PROTOCOL NEEDED! | ||
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[[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]] | [[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]] | ||
==More ''in vivo'' goodies== | ===More ''in vivo'' goodies=== | ||
[[Sauer:Competent cell preparation]] PROTOCOL NEEDED! | [[Sauer:Competent cell preparation]] PROTOCOL NEEDED! | ||
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[[Sauer:P1vir phage transduction]] | [[Sauer:P1vir phage transduction]] | ||
==Other good stuff== | [[Sauer:Plasmid-born λRed recombineering using dsDNA]] | ||
===Other good stuff=== | |||
[[Library Generation|Generation of genomic libraries]] | [[Library Generation|Generation of genomic libraries]] | ||
[[Sauer:HPLC protocol]] | |||
[[Sauer:FPLC protocol]] | |||
</div> | </div> | ||
Latest revision as of 14:34, 27 July 2009
Please link protocols to the general Protocols page if appropriate.
