From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Other good stuff)
Current revision (16:34, 27 July 2009) (view source)
(Protein Expression/Purification)
(3 intermediate revisions not shown.)
Line 14: Line 14:
[[Sauer:ClpP purification]]  
[[Sauer:ClpP purification]]  
[[Sauer:ClpA purification]]
[[Sauer:SspB purification]]
[[Sauer:SspB purification]]
Line 24: Line 26:
[[Sauer:GFP-H6-ssrA purification]]
[[Sauer:GFP-H6-ssrA purification]]
[[Sauer:GST-sFtsH purification]]
[[Sauer:FtsH purification]]
===''In vitro'' enzymatic assays===
===''In vitro'' enzymatic assays===
Line 34: Line 40:
[[Sauer:ATPase assays]]
[[Sauer:ATPase assays]]
[[Sauer:In vitro peptide degradation by ClpXP]]
===Proteins: ''in vitro''===
===Proteins: ''in vitro''===

Current revision


Home        Protocols        Lab Members        Materials        Equipment        Links        Internal       

Please link protocols to the general Protocols page if appropriate.

Protein Expression/Purification

Sauer:ClpX purification

Sauer:His6 ClpX purification

Sauer:ClpP purification

Sauer:ClpA purification

Sauer:SspB purification

Sauer:Expression/purification of 35S-Met proteins

Sauer:Purification of His6-tagged proteins

Sauer:Lysing E. coli with Lysozymes

Sauer:GFP-H6-ssrA purification

Sauer:GST-sFtsH purification

Sauer:FtsH purification

In vitro enzymatic assays

Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:In vitro degradation of GFP-ssrA by ClpAP

Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!

Sauer:ATPase assays

Sauer:In vitro peptide degradation by ClpXP

Proteins: in vitro

Sauer:Silver staining

Sauer:bis-Tris SDS-PAGE, the very best

Nucleic acids: in vitro

Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Quantitating nucleic acids

Sauer:Site-directed mutagenesis

Sauer:RNA Purification from E. coli

Sauer:Stitching Genes by PCR

Personal tools