Sauer:Protocols: Difference between revisions
Line 16: | Line 16: | ||
[[Sauer:Purification of His-tagged proteins|Sauer:Purification of His<sub>6</sub>-tagged proteins]] | [[Sauer:Purification of His-tagged proteins|Sauer:Purification of His<sub>6</sub>-tagged proteins]] | ||
[[Sauer:Lysing E. coli with Lysozymes]] | |||
===''In vitro'' enzymatic assays=== | ===''In vitro'' enzymatic assays=== |
Revision as of 14:30, 24 January 2007
Please link protocols to the general Protocols page if appropriate.
Protein Expression/PurificationSauer:Expression/purification of 35S-Met proteins Sauer:Purification of His6-tagged proteins Sauer:Lysing E. coli with Lysozymes In vitro enzymatic assaysSauer:In vitro degradation of GFP-ssrA by ClpXP Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED! Proteins: in vitroSauer:bis-Tris SDS-PAGE, the very best Nucleic acids: in vitroSauer:In vitro transcription with T7 RNA polymerase Sauer:Phenol/chloroform extraction Sauer:Nucleic acid quantitation Sauer:Site-directed mutagenesis Sauer:RNA Purification from E. coli
|
Analysis of proteins or RNA in vivoSauer:Western blot PROTOCOL NEEDED! Sauer:Quantitative Western blot PROTOCOL NEEDED! Sauer:Northern blot PROTOCOL NEEDED! Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED! Sauer:beta-Galactosidase Assay (A better Miller) More in vivo goodiesSauer:Competent cell preparation PROTOCOL NEEDED! Sauer:P1vir phage transduction Other good stuff |