Sauer:Protocols

SAUER LAB

Home        Protocols        Lab Members        Materials        Equipment        Links        Internal       

Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.

PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.

Protein Expression/Purification Sauer:ClpX purification Sauer:ClpP purification PROTOCOL NEEDED! Sauer:Expression/purification of 35S-Met proteins In vitro enzymatic assays Sauer:In vitro degradation of GFP-ssrA by ClpXP Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED! Sauer:ATPase assays PROTOCOL NEEDED! Proteins: in vitro Sauer:Silver staining Sauer:bis-Tris SDS-PAGE, the very best Nucleic acids: in vitro Sauer:In vitro transcription with T7 RNA polymerase Sauer:Phenol/chloroform extraction Sauer:Nucleic acid quantitation Sauer:Site-directed mutagenesis

Analysis of proteins or RNA in vivo Sauer:Western blot PROTOCOL NEEDED! Sauer:Quantitative Western blot PROTOCOL NEEDED! Sauer:Northern blot PROTOCOL NEEDED! Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED! Sauer:beta-Galactosidase Assay (A better Miller) More in vivo goodies Sauer:Competent cell preparation PROTOCOL NEEDED! Sauer:P1vir phage transduction Other good stuff Generation of genomic libraries