Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.
PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.
Sauer:ClpP purification PROTOCOL NEEDED!
In vitro enzymatic assays
Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!
Sauer:ATPase assays PROTOCOL NEEDED!
Proteins: in vitro
Nucleic acids: in vitro
Analysis of proteins or RNA in vivo
Sauer:Western blot PROTOCOL NEEDED!
Sauer:Quantitative Western blot PROTOCOL NEEDED!
Sauer:Northern blot PROTOCOL NEEDED!
Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED!
More in vivo goodies
Sauer:Competent cell preparation PROTOCOL NEEDED!
Other good stuff