Sauer:Purification of His-tagged proteins/Denaturing prep

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Revision as of 14:46, 8 February 2006 by Kathmc (talk | contribs) (Sauer:Purification of His-tagged proteins/Deanturing prep moved to Sauer:Purification of His-tagged proteins/Denaturing prep)
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This is a general protocol for purification of His6-tagged proteins under denaturing conditions. Note that you may need to modify this protocol to fit your specific needs.

Buffers

Lysis buffer

100 mM NaH2PO4

10 mM Tris base

6 M guanidine hydrochloride

10 mM imidazole

Adjust to pH 8

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