Sauer:Purification of His-tagged proteins/Native prep: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
=Introduction= | |||
This is a general protocol for purification of His<sub>6</sub>-tagged proteins under native conditions. Note that you may need to modify this protocol to fit your specific needs. | This is a general protocol for purification of His<sub>6</sub>-tagged proteins under native conditions. Note that you may need to modify this protocol to fit your specific needs. | ||
=Buffers= | =Buffers= | ||
Revision as of 16:07, 9 February 2006
Introduction
This is a general protocol for purification of His6-tagged proteins under native conditions. Note that you may need to modify this protocol to fit your specific needs.
Buffers
Lysis buffer
50 mM NaH2PO4
500 mM NaCl
10 mM imidazole
Adjust to pH 8
Wash buffer
50 mM NaH2PO4
500 mM NaCl
20 mM imidazole
Adjust to pH 8
Elution buffer
50 mM NaH2PO4
500 mM NaCl
250 mM imidazole
Adjust to pH 8
