Sauer:Silver staining

SAUER LAB

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Who to ask about this protocol

Eyal, Kathleen

The recommended volume of solutions for mini-gels is 50 mL

- 1hr (or more) 50% EtOH, 12%HOAc, 0.5 mL/L Formaldehyde (37%)

- 2 x 20min 50% EtOH

- 20 sec ddH₂O

- 1 min 0.2 g/L Na₂S₂O₃•5H₂O

- 3 x 20 sec ddH₂O

- 20 min 2 g/L AgNO₃, 750 µL/L Formaldehyde (37%)

- 2 x 20 sec ddH₂O

Prepare developing solutions in advance!

- 30 g/L K₂CO₃, 500 µL/L Formaldehyde (37%), 4 mg/L Na₂S₂O₃.5H₂O (for 50 mL just use the smallest granule you can find)

- Shake gently until developed (typically less than a minute)

- 1 min 1% Glycine

- 1 min ddH₂O

- 10 min 1% Glycine

- 30 min ddH₂O

• NOTE:Many of the silver stain solutions are hazardous waste and cannot go down the sink. Please dispose of them properly.

This is an example of how it should look: