|
|
| (71 intermediate revisions by 9 users not shown) |
| Line 1: |
Line 1: |
| [http://web.mit.edu/biology/sauerlab/ Official lab website]
| | {{Sauer lab sidebar2}} |
| | {| cellspacing="3" |
| | |- valign="top" |
| | |width=365px style="border: 1px solid #999999; color: #FFFFFF; background-color: #FFFFFF; text-align:center"| |
| | <div style="padding: 0px"> |
|
| |
|
| =Lab members on OWW= | | =<font size=6 font style="color:#000000">Lab Information</font>= |
| [[User:Kathmc|Kathleen McGinness]]
| |
|
| |
|
| [[Dan Bolon]] | | [[Sauer:Lab Members|<font size=4 font style="color:#000000">Lab Members</font>]] |
|
| |
|
| =Former lab members on OWW= | | [http://mit.edu/sauerlab/ <font size=4 font style="color:#000000">Official lab website</font>] |
|
| |
|
| [[User:Jimhu|Jim Hu]] | | [http://web.mit.edu/biology/www/facultyareas/facresearch/sauer.html <font size=4 font style="color:#000000">Bob Sauer's Department of Biology page</font>] |
|
| |
|
| =Protocols= | | =<font size=6 font style="color:#000000">Resources</font>= |
| Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general [[Protocols]] page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.
| | [[Sauer:Protocols|<font size=4 font style="color:#000000">Protocols</font>]] |
|
| |
|
| | [[Sauer:Links|<font size=4 font style="color:#000000">Links</font>]] |
|
| |
|
| '''PLEASE NOTE:''' Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.
| | [[Sauer:Internal|<font size=4 font style="color:#000000">Internal</font>]]</div> |
| | | |} |
| | | <div style="padding: 0px"></div> |
| [[Sauer:ClpX purification]] | | <wikionly>[http://sauer.openwetware.org/ Dewikified version of the Sauer lab page]</wikionly> |
| | |
| [[Sauer:ClpP purification]]
| |
| | |
| [[Sauer:In vitro degradation of GFP-ssrA by ClpXP]]
| |
| | |
| [[Sauer:Expression/purification of 35S-Met proteins]] | |
| | |
| [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]]
| |
| | |
| [[Sauer:ATPase assays]]
| |
| | |
| [[Sauer:Western blot]]
| |
| | |
| [[Sauer:Quantitative Western blot]]
| |
| | |
| [[Sauer:Site-directed mutagenesis]]
| |
| | |
| [[Sauer:Competent cell preparation]]
| |
| | |
| [[Sauer:P1vir phage transduction]]
| |
| | |
| [[Sauer:Northern blot]]
| |
| | |
| [[Sauer:Silver staining]]
| |
| | |
| [[Sauer:In vivo tmRNA tagging assays]]
| |
| | |
| =Equipment (Sauer lab)=
| |
| | |
| Please list here the equipment we have in our lab and the appropriate contact person for that piece of equipment. If it is something a lot of people use, also list other people that may be able to help out if there are any questions.
| |
| | |
| [[Thermocyclers (PCR machines)]]
| |
| | |
| [[HPLC]]
| |
| | |
| [[FPLC]]
| |
| | |
| [[Fluorimeter]]
| |
| | |
| [[Spectrometers]]
| |
| | |
| [[Plate reader]]
| |
| | |
| [[Analytical ultracentrifuge]]
| |
| | |
| [[CD spectrometer]]
| |
| | |
| [[Gel dryer]]
| |
| | |
| [[Developer]]
| |
| | |
| [[Sonicator]]
| |
| | |
| [[Milli-Q water system]]
| |
| | |
| [[Speedvac]]
| |
| | |
| [[Lyophilizer]]
| |
| | |
| [[Electroporator]]
| |
| | |
| =Equipment (other labs)=
| |
| | |
| Please list here equipment that we use in other labs along with a contact person in our lab and a contact person in the other lab. Tips on how reserving/using the equipment works would also be helpful i.e. do we need training on the equipment?.
| |
