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| [http://web.mit.edu/biology/sauerlab/ Official lab website]
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| =Lab members on OWW= | | =<font size=6 font style="color:#000000">Lab Information</font>= |
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| [[Dan Bolon]] | | [[Sauer:Lab Members|<font size=4 font style="color:#000000">Lab Members</font>]] |
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| [[user:cmf|Chris Farrell]] | | [http://mit.edu/sauerlab/ <font size=4 font style="color:#000000">Official lab website</font>] |
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| [[user:eyalg|Eyal Gur]] | | [http://web.mit.edu/biology/www/facultyareas/facresearch/sauer.html <font size=4 font style="color:#000000">Bob Sauer's Department of Biology page</font>] |
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| [[user:marylee|Mary Lee]] | | =<font size=6 font style="color:#000000">Resources</font>= |
| | [[Sauer:Protocols|<font size=4 font style="color:#000000">Protocols</font>]] |
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| [[user:amartin|Andreas Martin]] | | [[Sauer:Links|<font size=4 font style="color:#000000">Links</font>]] |
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| [[Kathleen McGinness]] | | [[Sauer:Internal|<font size=4 font style="color:#000000">Internal</font>]]</div> |
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| =Former lab members on OWW=
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| | | <wikionly>[http://sauer.openwetware.org/ Dewikified version of the Sauer lab page]</wikionly> |
| [[User:Jimhu|Jim Hu]]
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| =Protocols= | |
| Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general [[Protocols]] page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.
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| '''PLEASE NOTE:''' Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.
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| [[Sauer:ClpX purification]]
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| [[Sauer:ClpP purification]]
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| [[Sauer:In vitro degradation of GFP-ssrA by ClpXP]]
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| [[Sauer:Expression/purification of 35S-Met proteins]]
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| [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]]
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| [[Sauer:ATPase assays]]
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| [[Sauer:Western blot]]
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| [[Sauer:Quantitative Western blot]]
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| [[Sauer:Site-directed mutagenesis]]
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| [[Sauer:Competent cell preparation]]
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| [[Sauer:P1vir phage transduction]]
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| [[Sauer:Northern blot]]
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| [[Sauer:Silver staining]]
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| [[Sauer:In vivo tmRNA tagging assays]]
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| [[Sauer:In vitro transcription with T7 RNA polymerase]]
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| [[Sauer:Phenol/chloroform extraction]]
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| =Equipment (Sauer lab)=
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| Please list here the equipment we have in our lab and the appropriate contact person for that piece of equipment. If it is something a lot of people use, also list other people that may be able to help out if there are any questions.
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| [[Sauer:Thermocyclers (PCR machines)]]
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| [[Sauer:HPLC]]
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| [[Sauer:FPLC]]
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| [[Sauer:Fluorimeter]]
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| [[Sauer:Spectrometers]]
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| [[Sauer:Plate reader]]
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| [[Sauer:Analytical ultracentrifuge]]
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| [[Sauer:CD spectrometer]]
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| [[Sauer:Gel dryer]]
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| [[Sauer:Developer]]
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| [[Sauer:Sonicator]]
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| [[Sauer:Milli-Q water system]]
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| [[Sauer:Speedvac]]
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| [[Sauer:Lyophilizer]]
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| [[Sauer:Electroporator]]
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| =Equipment (other labs)=
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| Please list here equipment that we use in other labs along with a contact person in our lab and a contact person in the other lab. Tips on how reserving/using the equipment works would also be helpful i.e. do we need training on the equipment?.
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| [[Endy:Gel imager|Gel imager (Endy lab)]]
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| [[Bell:Smart system|Smart system (Bell lab)]]
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| [[Baker:Phosphorimager|Phosphorimager (Baker lab)]]
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| [[Baker:Fluorimager|Fluorimager (Baker lab)]]
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| =Other lab stuff=
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| [[Sauer:safety|Safety]]
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| [[Enzymes]]
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| [[Chemicals]]
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| =Miscellaneous links= | |
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| [http://web.mit.edu/ MIT website]
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| [https://webmail.mit.edu/ MIT webmail]
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| *Login required
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| [http://web.mit.edu/ecat/ MIT ECAT ordering] | |
| *Certificate required
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| [http://web.mit.edu/sapweb/PS1/index.shtml MIT SAPweb]
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| *Purchase orders, etc. Certificate required.
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