Sauer Lab
Lab members on OWW
Former lab members on OWW
Protocols
Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.
PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.
Sauer: In vitro degradation of GFP-ssrA by ClpXP
Sauer: Expression/purification of 35S-Met proteins
Sauer: In vitro degradation of radiolabeled proteins by ClpXP
Sauer: Quantitative Western blot
Sauer: Site-directed mutagenesis
Sauer: Competent cell preparation
Equipment (Sauer lab)
Please list here the equipment we have in our lab and the appropriate contact person for that piece of equipment. If it is something a lot of people use, also list others that may be able to help out if there are any questions.
Equipment (other labs)
Please list here equipment that we use in other labs along with a contact person in our lab and a contact person in the other lab. Tips on how reserving/using the equipment works would also be helpful i.e. do we need training on the equipment?.
