Sbb14-Dingguo Chen: Difference between revisions
Dingguo Chen (talk | contribs) No edit summary |
Dingguo Chen (talk | contribs) No edit summary |
||
| Line 6: | Line 6: | ||
==[[User:Dingguo Chen|Dingguo Chen]] 12:20, 6 March 2014 (PST)== | ==[[User:Dingguo Chen|Dingguo Chen]] 12:20, 6 March 2014 (PST)== | ||
Run gel for Ala, Leu, and | Run gel for soeing PCRs(Ala, Leu) and make sure they have the the correct size. | ||
Run gel to check size of simple PCR products for other amino acides, then do zymo cleanup. | |||
Results: | |||
All 4 soeing parts seem to have the correct sizes. | |||
for the normal PCRs, all but gul1 look good. | |||
==[[User:Dingguo Chen|Dingguo Chen]] 11:30, 11 March 2014 (PST)== | |||
Redo PCRs for Glu1 and ran gel for PCR(Ala,and Leu). | |||
Results: | |||
Soeing PCR on Ala doesn't work and need to redo. | |||
==[[User:Dingguo Chen|Dingguo Chen]] 12:20, 6 March 2014 (PST)== | |||
Run gel for Ala Soeing PCR-increase the concentration of the templates to 5 ul each this time. | |||
Results: | |||
We got PCR part that have the desired size. | |||
Plan for next lab: | |||
Do digestion and gel purification on all the PCR products. | |||
Revision as of 22:55, 16 March 2014
~~!~~
We did Soeing for Ala and Leu, which need combing the PCR products together. There's no product cloning out for Glu last time, so we are working on the PCR for these three today.
Dingguo Chen 12:20, 6 March 2014 (PST)
Run gel for soeing PCRs(Ala, Leu) and make sure they have the the correct size.
Run gel to check size of simple PCR products for other amino acides, then do zymo cleanup.
Results: All 4 soeing parts seem to have the correct sizes. for the normal PCRs, all but gul1 look good.
Dingguo Chen 11:30, 11 March 2014 (PST)
Redo PCRs for Glu1 and ran gel for PCR(Ala,and Leu). Results: Soeing PCR on Ala doesn't work and need to redo.
Dingguo Chen 12:20, 6 March 2014 (PST)
Run gel for Ala Soeing PCR-increase the concentration of the templates to 5 ul each this time. Results: We got PCR part that have the desired size. Plan for next lab: Do digestion and gel purification on all the PCR products.
