Sbb14-jpn: Difference between revisions
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We determined that because alanyl-tRNA synthetase is a homodimer that the other related sequence we had found (375 aa) is not another chain to the protein and unnecessary for our experiment. We also made a choice between the two aspartyl-tRNA synthetases for the one which is not of the nondescriminating variety. | We determined that because alanyl-tRNA synthetase is a homodimer that the other related sequence we had found (375 aa) is not another chain to the protein and unnecessary for our experiment. We also made a choice between the two aspartyl-tRNA synthetases for the one which is not of the nondescriminating variety. | ||
--[[User:Joshua P Nixon|Joshua P Nixon]] 13:30, 18 February 2014 (EST) | --[[User:Joshua P Nixon|Joshua P Nixon]] 13:30, 18 February 2014 (EST) | ||
Today we started the wetlab portion of our experiment by miniprepping the Pbad-Oled from E. Coli so that we may later clone our aminoacyl tRNA synthetase constructs into it (We use a Quiagen miniprep kit). --[[User:Joshua P Nixon|Joshua P Nixon]] 01:19, 28 February 2014 (EST) | |||
Revision as of 23:19, 27 February 2014
Today we created a design spreadsheetwhich we will use to design oligos for cloning our assigned aminoacyl tRNA synthetases--Joshua P Nixon 16:15, 14 February 2014 (EST)
We determined that because alanyl-tRNA synthetase is a homodimer that the other related sequence we had found (375 aa) is not another chain to the protein and unnecessary for our experiment. We also made a choice between the two aspartyl-tRNA synthetases for the one which is not of the nondescriminating variety. --Joshua P Nixon 13:30, 18 February 2014 (EST)
Today we started the wetlab portion of our experiment by miniprepping the Pbad-Oled from E. Coli so that we may later clone our aminoacyl tRNA synthetase constructs into it (We use a Quiagen miniprep kit). --Joshua P Nixon 01:19, 28 February 2014 (EST)
