Schwekendiek Lab Inventory

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Restriction Enzymes

Enzyme	Fast Digest	Supplier	Availability	Location	Last update
Acc65I (KpnI)	yes	Fermentas	in stock	Freezer 1	--Axel 21:08, 24 January 2007 (EST)
BamH1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
BglII	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Cla1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
EcoR1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
EcoRV	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
HindIII	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Kpn1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Nco1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Not1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Pst1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Sac1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Sal1	no	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Sma1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
SpeI (BcuI)	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Xba1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)
Xho1	yes	Fermentas	in stock	Freezer 1	--Axel 16:34, 18 March 2008 (CDT)

DNA Modifying Enzymes

Enzyme	Supplier	Order No.	Availability	Location	Last update
Taq DNA Polymerase	Fermentas	N/A	in stock	Freezer 1	--Axel 16:35, 18 March 2008 (CDT)
Taq DNA Polymerase	Invitrogen	N/A	in stock	Freezer 1	--Axel 16:25, 18 March 2008 (CDT)
Taq Master Mix	Denville	N/A	in stock	Freezer 2	--Axel 14:26, 19 March 2008 (CDT)
T4-DNA Ligase	Fermentas	N/A	in stock	Freezer 1	--Axel 16:35, 18 March 2008 (CDT)
DNAse I	Fermentas	N/A	in stock	Freezer 1	--Axel 16:35, 18 March 2008 (CDT)
M-MuLV Reverse Transcriptase	Fermentas	N/A	in stock	Freezer 1	--Axel 16:35, 18 March 2008 (CDT)

Bacterial Strains

Strain	Resistance	Location	Source	Last update
EHA101	KAN	Iceman	Dr. Walter	--Axel 16:24, 18 March 2008 (CDT)
LBA4404		Iceman	Dr. Matthews	--Axel 16:24, 18 March 2008 (CDT)
AGLO		Iceman	Dr. Abebe	--Axel 16:24, 18 March 2008 (CDT)
DH5-α	None	Iceman	Dr. Abebe	--Axel 21:08, 24 January 2007 (EST)
DB3.1	None	Iceman	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
Transbacter			Cambia (considered)	--Axel 21:08, 24 January 2007 (EST)

Antibiotic Stocks

Antibiotic	Concentration	Location	Supplier	Last update
Ampicillin	60 mg/ml	Freezer 1	Gold Biotech	--Axel 16:43, 18 March 2008 (CDT)
Kanamycin	50 mg/ml	Freezer 1	Gold Biotech	--Axel 16:43, 18 March 2008 (CDT)
Chloramphenicol	34 mg/ml	Freezer 1	Gold Biotech	--Axel 16:43, 18 March 2008 (CDT)
Spectomycin		Freezer 1	Gold Biotech	--Axel 16:43, 18 March 2008 (CDT)
Rifampicin	50 mg/ml	Freezer 1	Gold Biotech	--Axel 16:43, 18 March 2008 (CDT)
Carbenicillin		Freezer 1	Gold Biotech	--Axel 16:43, 18 March 2008 (CDT)

Plasmids

Plasmid	Host	Location	Source	Last update
pBluescript KS	DH5-α	Iceman:A1	Stratagene	--Axel 21:08, 24 January 2007 (EST)
P-DONR	N/A	Iceman:A1	Invitrogen	--Axel 16:50, 18 March 2008 (CDT)
P-GFP	N/A	Iceman:A1	Dr. Abebe (UNI)	--Axel 21:08, 24 January 2007 (EST)
pSB1A3	DB3.1	Iceman:A1	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
pSB1A7	DB3.1	Iceman:A1	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
pSB1A10	DB3.1	Iceman:A1	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
J52017	DB3.1	Iceman:A1	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
J61002	DB3.1	Iceman:A1	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
J61003	DB3.1	Iceman:A1	Dr. Lizarazo (MIT)	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA0380	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA0390	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1200	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1201	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1281Z	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1291Z	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1300	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1301	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1302	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1303	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1304	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1380	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1381Xa	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1381Xb	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1381Xc	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1381Z	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1390	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1391	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1391Xa	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1391Xb	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1391Xc	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1391Z	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA2200	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA2201	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA2300	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA2301	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1105.1	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1305.1	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA1305.2	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA0305.1	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)
pCAMBIA0305.2	DH5-α	Iceman:A1	CAMBIA	--Axel 21:08, 24 January 2007 (EST)

Electrophoresis

Item	Supplier	Availability	Last update
Top Vision LE GQ Agarose	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
6x SDS Loading Dye	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
6x Mass Ruler Loading Dye	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
6x Orange Dye	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
Fast Ruler low range ladder	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
1 kb ladder	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
O´Gene Ruler 100 bp plus	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
2x Loading Dye for RNA	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
RiboRuler RNA ladder low range	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)
RiboRuler RNA ladder high range	Fermentas	in stock	--Axel 21:08, 24 January 2007 (EST)

Labelling & Detection

Item	Supplier	Last update
Biotin Chromogenic Detection Kit	Fermentas	--Axel 21:08, 24 January 2007 (EST)
DecaLabel DNA Labelling Kit	Fermentas	--Axel 21:08, 24 January 2007 (EST)
SensiBlot Plus Nylon Membrane	Fermentas	--Axel 21:08, 24 January 2007 (EST)

Plant Tissue Culture Media

Item	Supplier	Order No.	Availability	Last update
B5 vitamins	Caisson Labs		in stock	--Axel 21:08, 24 January 2007 (EST)

Plant Hormone Stocks

Hormone	Concentration	Supplier	Last update
2,4-D	1 mg/ml	N/A	--Axel 16:21, 18 March 2008 (CDT)
Acetosyringone	400 mM	Caisson Labs	--Axel 14:37, 19 March 2008 (CDT)
GA3	1000 x	Caisson Labs	--Axel 16:21, 18 March 2008 (CDT)
IAA		N/A	--Axel 21:08, 24 January 2007 (EST)
NAA		N/A	--Axel 16:21, 18 March 2008 (CDT)
TDZ	1000 x	Caisson Labs	--Axel 21:08, 24 January 2007 (EST)

Sterile Plant Cultures

Plant	Genotype/Cultivar/Variety	Status	Last update
Humulus lupulus	Tettnanger	established	--Axel 21:08, 29 May 2007 (EST)

Chemicals

Item	Supplier	Availability	Last update
CTAB	N/A	in stock	--Axel 16:13, 18 March 2008 (CDT)
Chloroform/Isoamylalcohol	Fisher Scientific	in stock	--Axel 16:13, 18 March 2008 (CDT)
DMSO	N/A	in stock	--Axel 16:09, 18 March 2008 (CDT)
Ethidium bromide	Fisher Scientific	in stock	--Axel 16:13, 18 March 2008 (CDT)
IPTG	N/A	in stock	--Axel 16:13, 18 March 2008 (CDT)
PEG 8000	Fisher Scientific	in stock	--Axel 16:13, 18 March 2008 (CDT)
Phenol/TE	Fisher Scientific	in stock	--Axel 16:13, 18 March 2008 (CDT)
PVP	N/A	in stock	--Axel 16:13, 18 March 2008 (CDT)
X-Gal	N/A	in stock	--Axel 16:13, 18 March 2008 (CDT)

Instrumentation

Item	Availability	Last update
Gradient PCR Cycler	2	--Axel 21:08, 24 January 2007 (EST)
Laminar Flow Hood	2	--Axel 21:08, 24 January 2007 (EST)
Chemical Hood	1	--Axel 21:08, 24 January 2007 (EST)
Plant tissue culture incubators	3	--Axel 21:08, 24 January 2007 (EST)
Temporary Immersion System	40	--Axel 21:08, 24 January 2007 (EST)
Gel electrophoresis	4	--Axel 16:18, 18 March 2008 (CDT)
Power supplies	2	--Axel 21:08, 24 January 2007 (EST)
Bench-top centrifuge	1	--Axel 21:08, 24 January 2007 (EST)
Shaker Incubator with light bench	1	--Axel 21:08, 24 January 2007 (EST)
Balance 0.01g	1	--Axel 21:08, 24 January 2007 (EST)
Balance 0.001g	1	--Axel 21:08, 24 January 2007 (EST)
Gyro Twister	1	--Axel 16:18, 18 March 2008 (CDT)
Gyro Shaker	1	--Axel 16:18, 18 March 2008 (CDT)
pH-meter	1	--Axel 21:08, 24 January 2007 (EST)
-20 Freezer	2	--Axel 16:37, 18 March 2008 (CDT)
-80 Freezer	1	--Axel 21:08, 24 January 2007 (EST)

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Schwekendiek Lab Inventory

Contents

Restriction Enzymes

DNA Modifying Enzymes

Bacterial Strains

Antibiotic Stocks

Plasmids

Electrophoresis

Labelling & Detection

Plant Tissue Culture Media

Plant Hormone Stocks

Sterile Plant Cultures

Chemicals

Instrumentation

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