Sentelab:Notebook/gene synthesis/2010/09/17: Difference between revisions
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Burak Yilmaz (talk | contribs) (Autocreate 2010/09/17 Entry for Sentelab:Notebook/gene_synthesis) |
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== | ==gene synthesis by using 2-step pcr== | ||
* | * 1st PCR mixture | ||
#42.75 uL ddH2O7 | |||
#1ul gfp 1-11 oligos (pooled) or gfp 12-22 pooled oligos | |||
#1.25 ul dNTP mix(10mM) | |||
#5 ul 10X pfu polymerase buffer with MgSO4 | |||
#1 ul Pfu turbo hotstart polymerase | |||
*PCR conditions | |||
#95C for 2 min | |||
#95C for 30 sec | |||
#50C for 30 sec | |||
#72C for 1 min | |||
#72C for 2 min | |||
#4C unlimited time | |||
Revision as of 05:14, 17 September 2010
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gene synthesis by using 2-step pcr
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