Sentelab:Notebook/gene synthesis/2010/09/18: Difference between revisions
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#5 volumes of buffer PB added to volume of the PCR reaction and mixed | #5 volumes of buffer PB added to volume of the PCR reaction and mixed | ||
#A QIAquick column is plaed ina provided 2 ml collection tube | #A QIAquick column is plaed ina provided 2 ml collection tube | ||
#To bind DNA, the sample is applied to the QIAquick | #To bind DNA, the sample is applied to the QIAquick column and centrifuged for 45 sec. Flow trough is discarded and QIAquick column is placed back into the same tube. | ||
column and centrifuged for 45 sec. Flow trough is discarded and | #To wash 0,75 ml buffer PE is added to the QIAquick column and centrifuged for 45 sec .Flow through is discaded and QIAquick column is placed back into the same tube. | ||
QIAquick column is placed back into the same tube. | |||
#To wash 0,75 ml buffer PE is added to the QIAquick column and centrifuged for | |||
45 sec .Flow through is discaded and QIAquick column is placed back into the same tube. | |||
#The column is centrifuged in a 2ml collection tube for 1min | #The column is centrifuged in a 2ml collection tube for 1min | ||
#Each QIA quick column is placed in a clean 1.5 ml microcentrifuge tube. | #Each QIA quick column is placed in a clean 1.5 ml microcentrifuge tube. |
Revision as of 07:27, 17 September 2010
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=1st PCR product purification by PCR purification kit
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