Shreffler:Eosinophil Isolation
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(Difference between revisions)
(New page: '''Granulocyte Isolation''': # Under hood, spray down the buffy bag and scissors with 70% EtOH and wait until dry. Then cut oopen the buffy coat bag and pour blood into a 50 mL Falcon tube...) |
Current revision (13:29, 4 May 2011) (view source) |
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| - | + | ==Overview== | |
| - | # Under hood, spray down the buffy | + | |
| + | Isolation of eosinophils for migration studies in collaboration with Bryan Hurley | ||
| + | |||
| + | ==Materials== | ||
| + | |||
| + | * | ||
| + | |||
| + | ==Procedure== | ||
| + | |||
| + | # Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry. | ||
| + | # Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube. | ||
| + | |||
| + | ==Discussion== | ||
| + | [[Talk:{{PAGENAME}}|discuss this protocol]] | ||
| + | |||
| + | ==References== | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ==Contact== | ||
| + | *Who has experience with this protocol? | ||
| + | *[[Special:Emailuser/Wayne G. Shreffler|Email Wayne Shreffler through OpenWetWare]] | ||
| + | |||
| + | [[Category:ShreffLab]] | ||
| + | |||
| + | [[Category:Protocol]] | ||
| + | |||
| + | [[Category:Flow cytometry]] | ||
| + | |||
| + | [[Category:Immunology]] | ||
Current revision
Contents |
Overview
Isolation of eosinophils for migration studies in collaboration with Bryan Hurley
Materials
Procedure
- Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry.
- Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube.
Discussion
References
Contact
- Who has experience with this protocol?
- Email Wayne Shreffler through OpenWetWare
