Shreffler:Notebook/Alex Daily/2012/03/21: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==SDS-PAGE on peanut protein from Mt Sinai== | ||
* Insert content here... | * Insert content here... | ||
Revision as of 08:56, 22 March 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SDS-PAGE on peanut protein from Mt Sinai
Ran SDS-PAGE with Bert to gauge purity/protein concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3 mg/ml. Ran nanodrop to determine true (approximate) protein concentrations: Ara h 2: ~1 mg/ml Ara h 3: ~2 mg/ml Ara h 6: ~0.5 mg/ml For SDS-PAGE, we want to create a loading solution for each protein at a concentration of 0.5 µg/µl. Loading solution composition: Loaded the gel w/ 1 µg and 5 µg for each protein. Gel According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well.
|