Shreffler:Notebook/Alex Daily/2012/03/21: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==SDS-PAGE on peanut protein from Mt Sinai==
==SDS-PAGE on peanut protein from Mt Sinai==
* Insert content here...


Ran SDS-PAGE with Bert to gauge purity/protein concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3 mg/ml. Ran nanodrop to determine true (approximate) protein concentrations:
Ran SDS-PAGE with Bert to gauge protein purity/concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3 mg/ml. Ran nanodrop to determine approximate protein concentrations:


Ara h 2: ~1 mg/ml
*Ara h 2: ~1 mg/ml
Ara h 3: ~2 mg/ml
*Ara h 3: ~2 mg/ml
Ara h 6: ~0.5 mg/ml
*Ara h 6: ~0.5 mg/ml


For SDS-PAGE, we want to create a loading solution for each protein at a concentration of 0.5 µg/µl.
Made loading solutions for each protein as follows:
Loading solution composition:
 
*Arah h 2:
*Arah h 3:
*Arah h 6:


Loaded the gel w/ 1 µg and 5 µg for each protein.
Loaded the gel w/ 1 µg and 5 µg for each protein.


Gel
[[Image:Gel]]


According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well.
According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well.




Revision as of 09:04, 22 March 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

SDS-PAGE on peanut protein from Mt Sinai

Ran SDS-PAGE with Bert to gauge protein purity/concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3 mg/ml. Ran nanodrop to determine approximate protein concentrations:

  • Ara h 2: ~1 mg/ml
  • Ara h 3: ~2 mg/ml
  • Ara h 6: ~0.5 mg/ml

Made loading solutions for each protein as follows:

  • Arah h 2:
  • Arah h 3:
  • Arah h 6:

Loaded the gel w/ 1 µg and 5 µg for each protein.

File:Gel

According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Shreffler:Notebook/Alex_Daily/2012/03/21&oldid=593689"