Shreffler:Notebook/Alex Daily/2012/04/04: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 14: | Line 14: | ||
[[Category: | [[Category: ShreffLab Sarita]] | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 11:52, 4 April 2012
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Sarita BuffySarita received buffy coat from MGH (from Tues 4/3/12). I ficolled/counted (550 million PBMCs) and stripped ~400 million cells with lactic acid following Sarita's lactic acid stripping protocol. These were washed twice and resuspended in 800 µl RPMI. 400 µl were passively sensitized with serum drawn from PNOIT #965-03 (high IgE levels for Ara h 2 according to phadia results); the other 400 µl were sensitized with Sarita's own serum as a negative control for peanut-specific response. Other 150 million cells used in Sarita's plasmablast staining. | |
