Shreffler:Notebook/Staff Meetings/2010/05/07: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2010/05/07 Entry for Shreffler:Notebook/Staff_Meetings) |
|||
Line 11: | Line 11: | ||
===Agenda Items=== | ===Agenda Items=== | ||
* | * look up GI fellow working in Mucosal Immunology to put in touch with Bert | ||
* ALDH variation -- fresh cells | |||
** Bert's conclusions: | |||
*** ALDH1A2 was upregulated by CPE in mDC, but not as much as I've seen before at Mt Sinai. This probably explains the small effect of CPE on RA-sensitive T cell surface markers. | |||
*** No difference between these fresh cells and recent results with buffy coats. Therefore, low response to CPE is probably not due to buffy coat production. | |||
*** Could be the medium. Changed to new batch around the same time as when I had the one good experiment (done on 3/12/10, stained T cells on 3/18/10). So could be due to new lot of medium, or to the Pen/Strep, which is also different from the Pen/Strep used at Mt Sinai. | |||
*** Will test various media and Pen/Strep variants in culture with mDC and use ALDH1A2 expression/upregulation by CPE as readout. | |||
** Will also test lots of stem cell media | |||
====Previous Action Items==== | ====Previous Action Items==== |
Revision as of 07:12, 7 May 2010
ShreffLab Staff Meetings | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Replace with user/time stampAgenda Items
Previous Action ItemsOther Discussion
Things To Do
|