Shreffler:Notebook/Staff Meetings/2010/05/07: Difference between revisions

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* ALDH variation -- fresh cells
* ALDH variation -- fresh cells
** Bert's conclusions:
** Bert's conclusions:
*** ALDH1A2 was upregulated by CPE in mDC, but not as much as I've seen before at Mt Sinai. This probably explains the small effect of CPE on RA-sensitive T cell surface markers.
*** ALDH1A2 was upregulated by CPE in mDC, but not as much as I've seen before at Mt Sinai. This probably explains the small effect of CPE on RA-sensitive T cell surface markers.
*** No difference between these fresh cells and recent results with buffy coats. Therefore, low response to CPE is probably not due to buffy coat production.
*** No difference between these fresh cells and recent results with buffy coats. Therefore, low response to CPE is probably not due to buffy coat production.

Revision as of 07:15, 7 May 2010

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Agenda Items

  • look up GI fellow working in Mucosal Immunology to put in touch with Bert
  • ALDH variation -- fresh cells
    • Bert's conclusions:
      • ALDH1A2 was upregulated by CPE in mDC, but not as much as I've seen before at Mt Sinai. This probably explains the small effect of CPE on RA-sensitive T cell surface markers.
      • No difference between these fresh cells and recent results with buffy coats. Therefore, low response to CPE is probably not due to buffy coat production.
      • Could be the medium. Changed to new batch around the same time as when I had the one good experiment (done on 3/12/10, stained T cells on 3/18/10). So could be due to new lot of medium, or to the Pen/Strep, which is also different from the Pen/Strep used at Mt Sinai.
      • Will test various media and Pen/Strep variants in culture with mDC and use ALDH1A2 expression/upregulation by CPE as readout.
    • Will also test lots of stem cell media

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