Shreffler:Notebook/Staff Meetings/2010/06/04: Difference between revisions

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==Replace with user/time stamp==
==*'''[[User:Wayne G. Shreffler|Wayne G. Shreffler]] 14:39, 28 May 2010 (EDT)''':==


===Agenda Items===
===Agenda Items===
Alex's work
* JAX
**


*  
*PM
 
* R03
** Reviewed data from 5 experiments (3/5 look like measurable DHR response)
** tentatively looks as though DHR is suppressed by EDTA
** Alex to export data from FJ to tables to complete analysis
 
* Carbo
 
----
Bert's work


====Previous Action Items====
* Ben Medoff's PCR gives better results -- dramatically lower Ct values across the board
* Despite this, CPE response looks weak -- one new lot of StemSpan versus old
* somewhat stronger in X-vivo (n=1)
** ? of whether only CPE response or other pathways are also suppressed
** perhaps we need fresh CPE?
* some hope in a poor quality experiment that ALDH inducing activity is fractionating
* WS will contact Luda about getting a little aliquot


*
====Action Items====


* Wayne will talk to Ben about using qPCR more regularly
* Bert will compare LPS response
* run fraction screening experiment on Ben's machine
* repeat fraction screening experiment with pooled fractions
* get larger column for bigger run
* contact Steve about using HPLC
----
----


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Revision as of 11:56, 4 June 2010

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*Wayne G. Shreffler 14:39, 28 May 2010 (EDT):

Agenda Items

Alex's work

  • JAX
  • PM
  • R03
    • Reviewed data from 5 experiments (3/5 look like measurable DHR response)
    • tentatively looks as though DHR is suppressed by EDTA
    • Alex to export data from FJ to tables to complete analysis
  • Carbo

Bert's work

  • Ben Medoff's PCR gives better results -- dramatically lower Ct values across the board
  • Despite this, CPE response looks weak -- one new lot of StemSpan versus old
  • somewhat stronger in X-vivo (n=1)
    • ? of whether only CPE response or other pathways are also suppressed
    • perhaps we need fresh CPE?
  • some hope in a poor quality experiment that ALDH inducing activity is fractionating
  • WS will contact Luda about getting a little aliquot

Action Items

  • Wayne will talk to Ben about using qPCR more regularly
  • Bert will compare LPS response
  • run fraction screening experiment on Ben's machine
  • repeat fraction screening experiment with pooled fractions
  • get larger column for bigger run
  • contact Steve about using HPLC

Other Discussion


Things To Do
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