Shreffler:Notebook/Staff Meetings/2011/03/15

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2011/03/15 Entry for Shreffler:Notebook/Staff_Meetings)
(Replace with user/time stamp)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Replace with user/time stamp==
+
==15 March 2011==
===Agenda Items===
===Agenda Items===
-
*  
+
* EoE
 +
**  Early Risk factors (recruiting, nothing for lab)
 +
** Biomarkers
 +
*** EDN assays: SK will run comparison of two assays this week with AM
 +
*** Eotaxin has been difficult to measure in saliva in FL's experience; could use CBA platform for other samples
 +
*** AM will meet with MY -- ws to arrange
-
====Previous Action Items====
+
* Basophil
 +
** APC project
 +
*** SP showed data on DR kinetics by flow and localization from the Amnis run:
 +
***: DR increases over 18 hours in an IL-3 dependent fashion
 +
***: DR increases in activation dep manner, but decreases from 20' to 2 h
 +
***: FcERI MFI rapidly falls and staining may become clustered -- from a few images, not quantitative analysis yet
 +
***: cell area increases (and perhaps aggregation) with activation
 +
***: surface CD11b increases
-
*  
+
* Allergen Adjuvant
 +
** we hope to hear from proteonomics on new sequence information this week
 +
** BR will run zymosan experiment this week
-
----
+
* PM Baso
 +
** AM produced very nice anergy experiment -- key variable to prevent 'carry over' appears to be time; by waiting 90' prior to 2nd stim, controls are perfect
 +
**: experiment demonstrates a degree of pathway-specific, partially non-specific suppression; NP-BSA somewhat stronger anergy-inducing signal, despite weaker stim (DerpNP_06 2/25/11)
-
===Other Discussion===
+
* JAX
 +
** updated scripts for JAX to run as data comes in, note nec format for pcr data
 +
** AM to catch up on analysis this month
 +
** requesting that K at Hopkins adds comment column for lab notes
 +
** mAb cocktail being sent; new PMA/CaI stim sent by AM
-
*  
+
* PM baso
 +
** AM to finish analysis this week as study nearing point of analysis
 +
* Carboplatin
 +
**  no changes
-
----
+
* U19
 +
** BR to schedule a meeting with JM
-
===Things To Do===
 
-
#
+
 
 +
 
 +
 
 +
----

Revision as of 10:02, 15 March 2011

ShreffLab Staff Meetings Main project page
Previous entry      

15 March 2011

Agenda Items

  • EoE
    • Early Risk factors (recruiting, nothing for lab)
    • Biomarkers
      • EDN assays: SK will run comparison of two assays this week with AM
      • Eotaxin has been difficult to measure in saliva in FL's experience; could use CBA platform for other samples
      • AM will meet with MY -- ws to arrange
  • Basophil
    • APC project
      • SP showed data on DR kinetics by flow and localization from the Amnis run:
        DR increases over 18 hours in an IL-3 dependent fashion
        DR increases in activation dep manner, but decreases from 20' to 2 h
        FcERI MFI rapidly falls and staining may become clustered -- from a few images, not quantitative analysis yet
        cell area increases (and perhaps aggregation) with activation
        surface CD11b increases
  • Allergen Adjuvant
    • we hope to hear from proteonomics on new sequence information this week
    • BR will run zymosan experiment this week
  • PM Baso
    • AM produced very nice anergy experiment -- key variable to prevent 'carry over' appears to be time; by waiting 90' prior to 2nd stim, controls are perfect
      experiment demonstrates a degree of pathway-specific, partially non-specific suppression; NP-BSA somewhat stronger anergy-inducing signal, despite weaker stim (DerpNP_06 2/25/11)
  • JAX
    • updated scripts for JAX to run as data comes in, note nec format for pcr data
    • AM to catch up on analysis this month
    • requesting that K at Hopkins adds comment column for lab notes
    • mAb cocktail being sent; new PMA/CaI stim sent by AM
  • PM baso
    • AM to finish analysis this week as study nearing point of analysis
  • Carboplatin
    • no changes
  • U19
    • BR to schedule a meeting with JM







Personal tools