Shreffler:Notebook/Staff Meetings/2011/03/15: Difference between revisions
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== | ==15 March 2011== | ||
===Agenda Items=== | ===Agenda Items=== | ||
* | * EoE | ||
** Early Risk factors (recruiting, nothing for lab) | |||
** Biomarkers | |||
*** EDN assays: SK will run comparison of two assays this week with AM | |||
*** Eotaxin has been difficult to measure in saliva in FL's experience; could use CBA platform for other samples | |||
*** AM will meet with MY -- ws to arrange | |||
* Basophil | |||
** APC project | |||
*** SP showed data on DR kinetics by flow and localization from the Amnis run: | |||
***: DR increases over 18 hours in an IL-3 dependent fashion | |||
***: DR increases in activation dep manner, but decreases from 20' to 2 h | |||
***: FcERI MFI rapidly falls and staining may become clustered -- from a few images, not quantitative analysis yet | |||
***: cell area increases (and perhaps aggregation) with activation | |||
***: surface CD11b increases | |||
* | * Allergen Adjuvant | ||
** we hope to hear from proteonomics on new sequence information this week | |||
** BR will run zymosan experiment this week | |||
---- | * PM Baso | ||
** AM produced very nice anergy experiment -- key variable to prevent 'carry over' appears to be time; by waiting 90' prior to 2nd stim, controls are perfect | |||
**: experiment demonstrates a degree of pathway-specific, partially non-specific suppression; NP-BSA somewhat stronger anergy-inducing signal, despite weaker stim (DerpNP_06 2/25/11) | |||
* JAX | |||
** updated scripts for JAX to run as data comes in, note nec format for pcr data | |||
** AM to catch up on analysis this month | |||
** requesting that K at Hopkins adds comment column for lab notes | |||
** mAb cocktail being sent; new PMA/CaI stim sent by AM | |||
* PM baso | |||
** AM to finish analysis this week as study nearing point of analysis | |||
* Carboplatin | |||
** no changes | |||
* U19 | |||
** BR to schedule a meeting with JM | |||
---- | |||
Revision as of 07:02, 15 March 2011
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15 March 2011Agenda Items
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