Shreffler:Notebook/retinoic acid trans signaling/2009/05/20: Difference between revisions
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*'''[[User:Neisha A. Rivers|Neisha A. Rivers]] 15:02, 1 June 2009 (EDT)''': | *'''[[User:Neisha A. Rivers|Neisha A. Rivers]] 15:02, 1 June 2009 (EDT)''': | ||
Plasmid Purification: | Plasmid Purification: as per [[Shreffler:Plasmid_Purification|protocol]] | ||
( Note: This process is used for rapid purification of transfection grade plasmid DNA) | ( Note: This process is used for rapid purification of transfection grade plasmid DNA) | ||
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Measure the Concentration of DNA using a UV Specrophotometer: | |||
( Note: Info on using Specrophotometery) [http://www.icampus.ucl.ac.be/courses/SBIM2520/document/genemol/index/Machines/spectrophitachi/DNAconcentration.html] | |||
Sample 1x = 189.7 ng/ul ~ 171 mg of DNA | |||
Ratio = 260/280 = 1.69 | |||
Sample 2x = 112.3 ng/ul | |||
Ratio = 260/280 = 1.8 | |||
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Prepare Glycerol Stock of Bacteria Culture: | |||
1. Vortex the cell culture in 15 ml tube | |||
2. Prepare a 20% glycerol solution (80% water + 20% glycerol) | |||
3. Take 400 ul of glycerol solution and place it into a 1.5 ml tube) | |||
4. Add 600 ul of the cell culture, invert mixture up and down, then place in -80 degree freezer for storage | |||
Revision as of 14:13, 3 June 2009
Retinoic Acid Trans Signaling | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Plasmid Purification: as per protocol ( Note: This process is used for rapid purification of transfection grade plasmid DNA)
Measure the Concentration of DNA using a UV Specrophotometer: ( Note: Info on using Specrophotometery) [1] Sample 1x = 189.7 ng/ul ~ 171 mg of DNA Ratio = 260/280 = 1.69 Sample 2x = 112.3 ng/ul Ratio = 260/280 = 1.8
Prepare Glycerol Stock of Bacteria Culture: 1. Vortex the cell culture in 15 ml tube 2. Prepare a 20% glycerol solution (80% water + 20% glycerol) 3. Take 400 ul of glycerol solution and place it into a 1.5 ml tube) 4. Add 600 ul of the cell culture, invert mixture up and down, then place in -80 degree freezer for storage
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