Shreffler:Notebook/retinoic acid trans signaling/2009/05/20: Difference between revisions
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(Autocreate 2009/05/20 Entry for Shreffler:Notebook/retinoic_acid_trans_signaling) |
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*'''[[User:Neisha A. Rivers|Neisha A. Rivers]] 15:02, 1 June 2009 (EDT)''': | |||
Plasmid Purification: | |||
( Note: This process is used for rapid purification of transfection grade plasmid DNA) | |||
Kit: Qiagen: HiSpeed Plasmid Purification Maxi Kit | |||
A) Bacterial culture, harvest, and lysis | |||
1. Pellet 150 ml overnight LB culture at 6000 x g for 15 min at 4 degree C | |||
2. Homogeneously resuspend the bacterial pellet in 10 ml Buffer P1 | |||
3. Add 10 ml Buffer P2, mis throughly by vigoorously inverting 4-6 times, and incubate at room temperature for 5 min | |||
4. | |||
Revision as of 12:02, 1 June 2009
 Retinoic Acid Trans Signaling
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Plasmid Purification: ( Note: This process is used for rapid purification of transfection grade plasmid DNA)
A) Bacterial culture, harvest, and lysis 1. Pellet 150 ml overnight LB culture at 6000 x g for 15 min at 4 degree C 2. Homogeneously resuspend the bacterial pellet in 10 ml Buffer P1 3. Add 10 ml Buffer P2, mis throughly by vigoorously inverting 4-6 times, and incubate at room temperature for 5 min 4.
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