Shreffler:Notebook/retinoic acid trans signaling/2009/05/20: Difference between revisions
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1. Pellet 150 ml overnight LB culture at 6000 x g for 15 min at 4 degree C | 1. Pellet 150 ml overnight LB culture at 6000 x g for 15 min at 4 degree C | ||
2. Homogeneously resuspend the bacterial pellet in 10 ml Buffer P1 | 2. Homogeneously resuspend the bacterial pellet in 10 ml Buffer P1 | ||
3. Add 10 ml Buffer P2, mis throughly by vigoorously inverting 4-6 times, and incubate at room temperature for 5 min | |||
3. Add 10 ml Buffer P2, mis throughly by vigoorously inverting 4-6 times, and incubate at room | |||
temperature for 5 min | |||
4. | 4. | ||
Revision as of 12:04, 1 June 2009
 Retinoic Acid Trans Signaling
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Plasmid Purification: ( Note: This process is used for rapid purification of transfection grade plasmid DNA)
A) Bacterial culture, harvest, and lysis 1. Pellet 150 ml overnight LB culture at 6000 x g for 15 min at 4 degree C 2. Homogeneously resuspend the bacterial pellet in 10 ml Buffer P1 3. Add 10 ml Buffer P2, mis throughly by vigoorously inverting 4-6 times, and incubate at room temperature for 5 min 4.
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