Shreffler:Notebook/retinoic acid trans signaling/2009/06/02: Difference between revisions
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(Autocreate 2009/06/02 Entry for Shreffler:Notebook/retinoic_acid_trans_signaling) |
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= | *'''[[User:Neisha A. Rivers|Neisha A. Rivers]] 16:16, 3 June 2009 (EDT)''': | ||
Concentrating DNA: | |||
(Note: We will use Sample 2x, which has a concentration of 112.3 ng/ml and a volume of 540 ul) | |||
1. Split the volume into 2 tubes = 270 in each epp. tubes | |||
2. Add 3 ul of glycogan to each tube | |||
3. Add equal volumes of isopropanol to each tube (total of 30 ul of isopropanol to each tube) | |||
4. Incubate for 30 min at room temperature | |||
5. Spin for 15 min at max speed | |||
6. Remove the liquid carefully without disturbing the pellet with a pipet and place into another tube | |||
7. Wash t | |||
Revision as of 13:16, 3 June 2009
 Retinoic Acid Trans Signaling
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Concentrating DNA: (Note: We will use Sample 2x, which has a concentration of 112.3 ng/ml and a volume of 540 ul) 1. Split the volume into 2 tubes = 270 in each epp. tubes 2. Add 3 ul of glycogan to each tube 3. Add equal volumes of isopropanol to each tube (total of 30 ul of isopropanol to each tube) 4. Incubate for 30 min at room temperature 5. Spin for 15 min at max speed 6. Remove the liquid carefully without disturbing the pellet with a pipet and place into another tube 7. Wash t
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