Shreffler:Notebook/retinoic acid trans signaling/2009/06/02

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2009/06/02 Entry for Shreffler:Notebook/retinoic_acid_trans_signaling)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
*'''[[User:Neisha A. Rivers|Neisha A. Rivers]] 16:16, 3 June 2009 (EDT)''':
-
* Insert content here...
+
 
 +
Concentrating DNA:
 +
 
 +
(Note: We will use Sample 2x, which has a concentration of 112.3 ng/ml and a volume of 540 ul)
 +
 
 +
1. Split the volume into 2 tubes = 270 in each epp. tubes
 +
 
 +
2. Add 3 ul of glycogan to each tube
 +
 
 +
3. Add equal volumes of isopropanol to each tube (total of 30 ul of isopropanol to each tube)
 +
 
 +
4. Incubate for 30 min at room temperature
 +
 
 +
5. Spin for 15 min at max speed
 +
 
 +
6. Remove the liquid carefully without disturbing the pellet with a pipet and place into another tube
 +
 
 +
7. Wash t

Revision as of 15:16, 3 June 2009

Retinoic Acid Trans Signaling Main project page
Previous entry      Next entry

Concentrating DNA:

(Note: We will use Sample 2x, which has a concentration of 112.3 ng/ml and a volume of 540 ul)

1. Split the volume into 2 tubes = 270 in each epp. tubes

2. Add 3 ul of glycogan to each tube

3. Add equal volumes of isopropanol to each tube (total of 30 ul of isopropanol to each tube)

4. Incubate for 30 min at room temperature

5. Spin for 15 min at max speed

6. Remove the liquid carefully without disturbing the pellet with a pipet and place into another tube

7. Wash t



Personal tools