Shreffler:Notebook/retinoic acid trans signaling/2009/07/30: Difference between revisions
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(Autocreate 2009/07/30 Entry for Shreffler:Notebook/retinoic_acid_trans_signaling) |
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== | =='''[[User:Neisha A. Rivers|Neisha A. Rivers]]'''== | ||
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Measuring Luciferase | |||
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* Transfer cells to 1.5 ml eppendorfs | |||
* Spin cells down for 500g for 10 min | |||
* Aspirate supernatants, then added 125 ul of PLB buffer, vortex | |||
* Allow to shake at room temperature for 30 min | |||
* Spin cells at 1400g for 2 min, leave supernatants | |||
* Add 25 ul of substrate (LAR) and 5 ul of lysate (supernatants) | |||
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Results: Luciferase was not detected in any of the samples | |||
Next Steps: Beta-gal assay, and a different transfection method - Lipofectamine | |||
Revision as of 11:52, 30 July 2009
 Retinoic Acid Trans Signaling
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Neisha A. RiversMeasuring Luciferase
Results: Luciferase was not detected in any of the samples Next Steps: Beta-gal assay, and a different transfection method - Lipofectamine
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