Shreffler:RBC Lysis Buffer for Eos: Difference between revisions
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(New page: RBC Lysis Buffer recipe (for Eosinophil protocol) **Procedure** # Measure out 500 ml DI water in beaker (slightly less is OK). # Bring solution to 0.8% (4 g) NH4Cl (Ammonium Chloride, abo...) |
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RBC Lysis Buffer recipe (for Eosinophil protocol) | RBC Lysis Buffer recipe (for Eosinophil protocol) | ||
==Procedure== | |||
# Measure out 500 ml DI water in beaker (slightly less is OK). | # Measure out 500 ml DI water in beaker (slightly less is OK). | ||
# Bring solution to 0.8% (4 g) NH4Cl (Ammonium Chloride, above Bert’s bench). | # Bring solution to 0.8% (4 g) NH4Cl (Ammonium Chloride, above Bert’s bench). | ||
Revision as of 10:53, 19 December 2011
RBC Lysis Buffer recipe (for Eosinophil protocol)
Procedure
- Measure out 500 ml DI water in beaker (slightly less is OK).
- Bring solution to 0.8% (4 g) NH4Cl (Ammonium Chloride, above Bert’s bench).
- Bring solution to 0.1 mM EDTA (100 µl of 0.5 M EDTA stock).
- Bring pH to 7.2 using 2M KHCO3 (Potassium Bicarbonate, in 50 ml conical above Bert’s bench, but if more see recipe below).
- Filter in 500 ml flask.
- For 2 M KHCO3, add 5 g KHCO3 to 25 ml DI water in 50 ml conical tube.
