Shreffler:Standard Buffers Solutions Media

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Many of these are available at Materials and to reduce redundancy those are referred to and this page is not included in the Protocols or Materials categories.

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Phosphate Buffered Saline (PBS)



Luria Bertani Media

Modified to make 0.5 L

  • Dissolve:
    • 5g of tryptone,
    • 2.5g of yeast extract, and
    • 5g of NaCl in 450 mL of dH2O
  • Place on stirring plate with stir bar and allow to mix, until everything dissolves
  • After completely dissolved, bring the volume up to 500 mL
  • Divide into two 150 ml flasks and store remaining in a sterile bottle
  • Autoclave the two flasks and sterile bottle (30 min)


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