Shreffler:Standard Buffers Solutions Media

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Media

LB

Luria Bertani Media

Modified to make 0.5 L

• Dissolve:
  • 5g of tryptone,
  • 2.5g of yeast extract, and
  • 5g of NaCl in 450 mL of dH₂O
• Place on stirring plate with stir bar and allow to mix, until everything dissolves
• After completely dissolved, bring the volume up to 500 mL
• Divide into two 150 ml flasks and store remaining in a sterile bottle
• Autoclave the two flasks and sterile bottle (30 min)