Silver: 50x TAE: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(categories) |
||
(One intermediate revision by one other user not shown) | |||
Line 1: | Line 1: | ||
50x TAE is 2M Tris, 2M Acetic Acid | The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA. | ||
Per 1L: | Per 1L: | ||
*242g Tris base | *242g Tris base | ||
*57.1 ml acetic acid | *57.1 ml acetic acid (glacial) | ||
*100ml 0.5 M EDTA | *100ml 0.5 M EDTA | ||
'''DO NOT AUTOCLAVE''' | '''DO NOT AUTOCLAVE''' | ||
[[Category:Material]] | |||
[[Category:Buffer]] | |||
[[Category:Agarose gel electrophoresis]] |
Revision as of 06:02, 5 May 2009
The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.
Per 1L:
- 242g Tris base
- 57.1 ml acetic acid (glacial)
- 100ml 0.5 M EDTA
DO NOT AUTOCLAVE